HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Forsum, E. Articles by Siddiqi, I. H. Search for Related Content PubMed Articles by Forsum, E. Articles by Siddiqi, I. H.

Large-Scale Fractionation of Whey Protein Concentrates

Department of Nutrition, University of Uppsala, Box 551, S-751 22 Uppsala 1 Sweden

ABSTRACT

Large-scale fractionation of whey protein concentrates was performed by pre-paratory gel filtration. A prototype of a stacked column, KS 450, packed with Sephadex^® G-75 and equilibrated with .1 M phosphate buffer pH 6.3 was utilized. Both an ultrafiltered and a gel-filtered whey protein concentrate were fractionated into three different fractions, the protein composition of which was evaluated by electrophoresis, amino acid analyses, and casein estimations. The nitrogen distribution between the different fractions was also analyzed. By large-scale gel filtration, ß-lactoglobulin of high purity and preparations rich in -lactalbumin could be obtained in large quantities.