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Journal of Dairy Science Vol. 57 No. 2 156-159
© 1974 by American Dairy Science Association ®
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Measuring Unsaturation in Milkfat and Other Oils by Differential Infrared Spectroscopy

B. A. Anderson, R. Miller and M. J. Pallansch

Dairy Products Laboratory, Agricultural Research Service, U. S. Department of Agriculture, Washington, D. C. 20250

ABSTRACT

Infrared absorbance at 3.3µ was directly related to the iodine value of milk-fat samples with unusual unsaturation when a totally saturated analog was a reference. Unsaturation was determined from differential infrared absorbance using a calibration curve. This technique was suitable for seven natural vegetable oils but not castor oil or cod-liver oil. The calibration curve was not applicable to hydrogenated oils because of the decrease in intensity due to isomeri-zation (cis-trans) of the olefinic bond.






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Copyright © 1974 by the American Dairy Science Association ®.