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Journal of Dairy Science Vol. 57 No. 1 56-60
© 1974 by American Dairy Science Association ®
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Desaturation and Saturation of Fatty Acids by Sheep Rumen Bacteria: Optimal Conditions and Cofactor Requirements

D. Sklan and P. Budowski

Faculty of Agriculture, The Hebrew University, Rehovot, Israel

ABSTRACT

Optimal conditions for aerobic formation and anaerobic biohydrogenation of linoleic acid were determined in sheep rumen fluid with added olive oil fatty acids. Both activities were highest at 38 C. Formation of linoleic acid exhibited maxima at pH 5.0 and 7.5, and hydrogenating activity was highest at pH 6.0.

Washed rumen bacteria suspended in phosphate buffer showed a requirement for reduced pyridine nucleotides in the aerobic formation of linoleic acid. Cysteine, quinol, and sucrose enhanced anaerobic hydrogenation and induced saturation of linoleic acid even under aerobic conditions. Addition of l-14carbon labeled oleic and linoleic acid revealed a bidirectional flux of label between the monoene and diene fraction upon aerobic incubation.






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Copyright © 1974 by the American Dairy Science Association ®.