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Journal of Dairy Science Vol. 56 No. 8 1095-1097
© 1973 by American Dairy Science Association ®
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Simple Technique for Preparing High Purity {alpha}-Lactalbumin1

R. L. Richter2, C. V. Morr and G. A. Reineccius

Department of Food Science and Nutrition, University of Minnesota, St. Paul 55101

ABSTRACT

A simple technique is described for preparing highly purified {alpha}-lactalbumin by adding 30% (weight/volume) polyethylene glycol 6000 to acid whey and fractionating the nonprecipitated proteins on DEAE Sephadex A-25. Disc. acrylamide gel electrophoresis confirmed the bimodal elution of highly purified {alpha}-lactalbumin from the column.

FOOTNOTES

1 Scientific Journal Series Paper No. 8139, Minnesota Agricultural Experiment Station, St. Paul 55101.

2 Present address: Department of Dairy Science, University of Florida, Gainesville 32601.






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Copyright © 1973 by the American Dairy Science Association ®.