JDS
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Journal of Dairy Science Vol. 56 No. 6 757-762
© 1973 by American Dairy Science Association ®
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gil, L. A.
Right arrow Articles by Moore, J. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gil, L. A.
Right arrow Articles by Moore, J. E.

Effect of Methionine Hydroxy Analog on Growth, Amino Acid Content, and Catabolic Products of Glucolytic Rumen Bacteria in Vitro1

L. A. Gil2, R. L. Shirley and J. E. Moore

Department of Animal Science, University of Florida, Gainsville 32601

ABSTRACT

Glucose was fermented by rumen bacteria utilizing urea for nitrogen with or without methionine hydroxy analog. The analog in excess of the bacterial requirement did not support additional growth after exhaustion of glucose. This indicated that growth stimulation by methionine hydroxy analog was not due to it as a source of energy. Its maximum effect on growth was at .2 mg/ml of medium. The free acid and calcium salt of methionine hydroxy analog and DL-methionine exhibited equivalent stimulation on bacteria. They disappeared from the medium in similar quantities at the time of accelerated growth. The analog had no effect on amino acid composition of bacterial protein. Some changes in amino acid composition during growth on glucose substrate indicated a shift in bacterial species. No methionine was in the cell-free medium. In a medium buffered at pH 6.9, lactate was the principal product of glucose fermentation. It increased at a rapid rate during the first 8 h during which acetate decreased proportionately on a molar percent basis. Bacteria grown with glucose retained from 50 to 65% of their initial cellulolytic capacity.

FOOTNOTES

1 Published by University of Florida, Institute of Food and Agricultural Sciences, University of Florida, Journal Series 4760.

2 Present address: Universidad Nacional de Colombia, Bogota, Colombia.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1973 by the American Dairy Science Association ®.