HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Richmond, P. A. Articles by Hood, L. F. Search for Related Content PubMed PubMed Citation Articles by Richmond, P. A. Articles by Hood, L. F.

Tissue Culture Method for Bovine Mammary Tissue: Lactose Synthesis

Department of Food Science, Cornell University, Ithaca, New York 14850

ABSTRACT

An in vitro method for investigating lactose synthesis in bovine mammary tissue is described. Tissue bits were incubated in an oxygen-enriched Medium 199 supplemented with bovine serum, antibiotics, hydroxycortisone acetate, prolactin, insulin, and U-¹⁴C-glucose or U-¹⁴C-galactose for 2, 12, 24, and 36 h. The tissue and medium were extracted separately with chloroform:methanol and radioactive components were identified. The mean percent recovery of radioactivity was 87 and 75% in the glucose and galactose experiments. After 36 h, 37 and 18% of the total radioactivity recovered was in lactose synthesized from glucose and galactose.

This method incorporates the advantages of the cell culture and tissue slice methods.