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Interference of Some Pesticides in the Milk Phosphatase Pasteurization Test

Department of Microbiology, Panjab University, Chandigarh-14, India

ABSTRACT

Some pesticides in milk interfere with the phosphatase test. Baygon and sevin formed a blue color with the Folin-Ciocalteu phenol reagent in milk. 1-Naphthol, a degradation product of sevin, had the same effect in pasteurized milk. Dimethylcarbamates decreased phenol formation in raw milk, but in pasteurized and in under-pasteurized milk a true phosphatase test was obtained. Tetramethyl thiuram disulphide at 25 µg/.5 ml and at higher concentrations influenced the test of under-pasteurized milk. Phosphamidon and 0:0 Dimethyl 2:2 dichlorovinyl phosphate at 5 µg/.5 ml gave phenol values in under-pasteurized milk equivalent to that in raw milk. Twelve pesticides decreased phenol formation in raw milk.