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Journal of Dairy Science Vol. 56 No. 4 419-422
© 1973 by American Dairy Science Association ®
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A Proteolytic Unit for Chymosin (Rennin) Activity Based on a Reference Synthetic Peptide

M. N. Raymond1, E. Bricas1, R. Salesse2, J. Garnier2,3, P. Garnot4 and B. Ribadeau Dumas4

Universite de Paris-Sud, Centre d'Orsay, France
I.N.R.A., Jouy-en-Josas, France

3 To whom to address correspondence.

ABSTRACT

The hexapeptide HLeu-Ser-Phe(NO2)-NIe-AIa-Leu-Ome is proposed as a reference for proteolytic activity determinations of chymosin (rennin). This peptide which is readily hydrolyzed by the enzyme contains a spectroscopic probe Phe (NO2) which facilitates routine determination of enzyme activity. Specific proteolyhc activity has been measured for various purified and commercial enzyme preparations and compared to their clotting activity.

FOOTNOTES

1 Laboratoire des peptides, E. R. n° 15, C.N.R.S., Institut de Biochimie.

2 Laboratoire de Biochimie Physique, I.N.R.A., Service de Biologie Physicochimique, Université de Paris-Sud, 91405 Orsay, France.

4 Laboratoire de Recherches sur les Protéines, Département de Technologie Animale, I.N.R.A.






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Copyright © 1973 by the American Dairy Science Association ®.