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Motility of Spermatozoa and Control of Bacteria in Bovine Semen Diluents Containing Penicillin and Neomycin or Epicillin¹

Dairy Breeding Research Center, Department of Dairy Science, The Pennsylvania State University, University Park 16802

ABSTRACT

In search for a suitable replacement for streptomycin, we determined effects of neomycin and epicillin upon sperm motility and control of bacteria in diluted bovine semen. Sperm livability at 5 C did not differ significantly for penicillin (units) and neomycin (micrograms) combinations of 0, 500, 1,000, and 1,500 of each per milliliter of skim-milk diluent. Each penicillin-neomycin combination effectively controlled bacterial growth during a 16-day storage. Epicillin alone at 0, 75, 150, 300, 600, and 1,200 micrograms per milliliter of skimmilk diluent did not significantly affect sperm motility; however, none efficiently retarded bacterial growth. A combination of penicillin and neomycin seems a satisfactory substitute for penicillin and streptomycin for controlling bacterial growth in diluted bull semen without impairing sperm livability.

¹ Authorized for publication as Paper 4246 in the journal series of the Pennsylvania Agricultural Experiment Station, University Park on 20 June 1972.

² Present address: Atlantic Breeders Cooperative, 1575 Apollo Drive, Lancaster, Pennsylvania 17601.