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Effect of Pesticides on the Methylene Blue Reduction Test in Milk

Department of Microbiology, Panjab University, Chandigarh, India

ABSTRACT

The effect of various pesticides on the methylene blue reduction test of milk was studied. Phosphamidon (0-(2-Chloro-2(diethylcarbamoyl)-1-methyl vinyl)-0-0-dimethyl phosphate), DDVP(0:0-dimethyl-2:2-dichlorovinyl phosphate), dimetilan (2-dimethyl carbamoyl-3-methyl pyrazolyl-(5)-dimethyl carbamate) , 1-naphthol, NaDDC (sodium diethyl-dithiocarbamate), baygon (2-isopropoxyphenyl-N-methyl carbamate), isolan (1-isopropyl-3-methylprazolyl-(5)-dimethyl carbamate), pyramat (2-propyl pyrimidyl-(6)-dimethyl carbamate), and amidithion (0,0-dimethyl-S-(2-methoxyethyl carbamoyl-methyl) phosphorodithioate) in raw milk did not affect the reduction time compared to the control. Sevin (1-naphthyl-N-methyl carbamate) and pyrolan (1-phenyl-3-methyl pyrazolyl-(5)-dimethyl carbamate) in milk increased the reduction time slightly but not significantly. Thiram (Tetramethylthiuram disulphide, TMTD) increased the reduction time at .05 µg/ml or above. But this pesticide did not significantly affect bacterial growth in milk.