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Separation of Lambda Casein and Some of Its Properties¹

Agricultural Products Utilization Biochemistry, Department of Animal and Range Sciences, Montana State University, Bozeman 59715

ABSTRACT

Crude -casein was prepared by extracting a 2.5% solution of iso-electric casein, half saturated with ammonium sulfate with N,N-dimethylformamide. The product was fractionated by column chromatography, and the -casein fraction which was associated with _s-casein was further purified by preparative electrophoresis. The -casein fraction contained nine components shown electrophoretically. It had a smaller number of amino acid residues than did _s1-casein based on a comparative molecular weight of 24,000. It was also richer in phosphorus ( 1.32%) than _s1-casein ( 1.08%). Its carbohydrate content was 3.94, 2.83, and 4.29% hexose, hexosamine, and sialic acid in comparison to _s1-casein which contained only traces of these.

¹ Contribution from Montana State University, Agricultural Experiment Station, Project No. 168, Paper No. 407.