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Effect of Glucose and Insulin on Lipoprotein Lipase Activity in Adipose Tissue and Milk¹

Department of Animal and Dairy Sciences, Agricultural Experiment Station, Auburn University, Auburn, Alabama 36830

ABSTRACT

Twelve pairs of Holstein cows were infused intravenously every 7th day with 3 liters of .9% saline or 25% glucose-saline for 24-h periods in a double reversal experiment to study the effect of glucose on lipoprotein lipase activity in adipose tissue and milk. The mean enzyme activities (µeq of fatty acids released per h per g of tissue or per ml of milk) for cows during saline and glucose treatments were: 22.2 and 26.8 in the adipose tissue and 332 and 342 in milk. Blood glucose of saline and glucose-infused cows was 54.6 and 96.9 mg/100 ml. Both blood glucose and lipoprotein lipase activity in adipose tissue were higher during glucose treatment than during saline treatment and were correlated .31. After the adipose tissue biopsy at the end of the infusion, six cows received 40 to 80 IU of insulin intravenously. Lipoprotein lipase activity in adipose tissue taken 15 min after insulin injection was 31 compared to 23 in the pre-insulin samples.

Adipose tissue samples from two non-lactating Holstein cows were incubated for 2.5 h at 38 C in: (a) 5 ml buffer, (b) buffer + 10 mg glucose, (c) buffer + 50 mil insulin, and (d) buffer + 10 mg glucose + 50 mU insulin. At the end of die incubation the mean lipoprotein lipase activities for treatments a, b, c, and d were 4.6, 7.7, 9.3, and 10.3.

¹ This report is a part of the thesis submitted by the senior author to the Graduate School of Auburn University in partial fulfillment of the requirements for the Ph. D. degree.

² Present address: Department of Dairy Science, University of Illinois, Urbana 61801.

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