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Electrophoretic Characterization of Bovine Serum Lipoproteins Throughout Gestation and Lactation^1,2,3,

Lipids Laboratory, The Pennsylvania State University, University Park 16802

ABSTRACT

The electrophoretic characteristics of the serum lipoproteins of Holstein cows were studied throughout gestation and lactation. The serum lipoproteins were separated into four ultracentrifugal density classes: very low density lipoproteins and chylomicrons < 1.006, low density lipoproteins 1.006 to 1.040, high density lipoproteins sub one 1.040 to 1.063, and high density lipoproteins 1.063 to 1.21. Agarose and polyacrylamide gel electrophoresis were used to analyze the lipoprotein fractions. The electrophoretic patern of blood serum was characterized by a dominant alpha band, a weak beta band, and no detectable pre-beta band.

Agarose electrophoresis of the ultra-centrifugal density classes demonstrated the association of very low density lipoproteins with pre-beta mobility, low density lipoproteins with beta mobility, and the association of high density lipoproteins sub one and high density lipoproteins with alpha mobility. There was an overlap in density between the lipoproteins demonstrating beta mobility and those with alpha mobility that was unresolvable based on ultracentrifugal separation.

Polyacrylamide gel electrophoresis revealed two bands of alpha mobility in the high density lipoproteins sub one throughout lactation, whereas only one alpha band was detected during nonlac-tation.

¹ Authorized for publication on May 16, 1973 as Paper No. 4449 in the Journal Series of the Pennsylvania Agricultural Experiment Station.

² Presented at the 1972 Annual Meeting of the American Dairy Science Association, Blacksburg, Virginia.

³ Supported in part by the U. S. Public Health Service, Research Grant HL 03632.

⁴ Present address: Medical School, University of California-San Diego, La Tolla, California 92037.