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Fractionation of Buffalo Milk Casein by Acrylamide Gel Electrophoresis and DEAE Cellulose Column Chromatography

Central Research Laboratory, Morinaga Milk Industry Co., Ltd., Tokyo, Japan
and National Dairy Research Institute, Karnal, India

ABSTRACT

Buffalo milk casein was separated in main three components, _s-, ß- and _K-caseins by acrylamide gel electrophoresis in the presence of 2-mercaptoethanol. Buffalo _s-casein had a slightly slower mobility than bovine _s-casein and revealed three or four accompanying bands. Buffalo and bovine ß-caseins have the same mobility. Buffalo _Kcasein revealed two bands which have been named earlier, _K-casein A and B, the B-band being more prominent. Buffalo _s-, ß- and _K-caseins were isolated by DEAE cellulose column chromatography and by gel electrophoresis to determine their homogeneity and their amino acid composition. Buffalo _s-casein fraction obtained by DEAE cellulose column chromatography revealed three bands on gel electrophoresis and _K-casein fraction revealed two bands. Buffalo ß-casein fraction was homogeneous. The amino acid composition of buffalo casein resembled that of bovine casein.