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Substitution of Filter Paper for Crucibles in the in Vitro Rumen True Digestibility Determination

Department of Animal Science, Cornell University, Ithaca, New York 14850

ABSTRACT

Filtration of the residue from the in vitro rumen true digestibility determination was attempted on filter paper. In the first experiment, comparisons were between in vitro true and apparent digestibility and between filter paper and sinter glass crucibles. There were significant (P<.05) differences between techniques of digestibility measurement and between the two filtration methods. In both techniques, filter paper gave a lower (P<.05) estimate of digestibility. Blank fermentations were run for the in vitro apparent digestibility estimate but not for the true digestibility estimate. This could explain the lowered in vitro true digestibility estimate when filter paper was used.

A second experiment, consisting of a series of in vitro true digestibilities with concurrent blank fermentations, was conducted. When the estimates using filter paper were corrected for the blank fermentations, they were not significantly different from those with crucibles.

The results suggest that filter paper can be substituted for crucibles in the estimation of in vitro rumen true digestibility provided a blank fermentation is conducted concurrently.

¹ Present address: Escuela Para Graduados En Ciencias Agropecurias, Balcarce, Argentina.