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Journal of Dairy Science Vol. 55 No. 8 1160-1164
© 1972 by American Dairy Science Association ®
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Ration Effect on Polyethylene Glycol as a Rumen Marker1

J. L. Clark, F. G. Hembry2, G. B. Thompson and R. L. Preston3

Department of Animal Husbandry, University of Missouri, Columbia, 65201

ABSTRACT

Three in vivo studies determined rate of passage of polyethylene glycol (PEG) from the rumen of sheep fed either ground alfalfa hay or a ration containing 4% cottonseed hulls (CSH) . Total rumen contents were collected immediately after slaughter, weight and volume determined, and samples collected for PEG and dry matter analysis. It appeared that rate of passage of PEG from the rumen of sheep was relatively constant (r= –.88, Reg. Coef. = –.85) when alfalfa was fed. However, when sheep were fed CSH, approximately 20% of the PEG was recovered 2 to 4 hr after PEG was administered to the sheep (r= –.56, Reg. Coef. = –.16). This suggested a rapid loss of PEG from the rumen or retention of PEG by nunen contents that interfered with analytical procedures.

In vitro studies determined if PEG was being retained by animals fed CSH. Approximately 91% of the PEG added to rumen contents of sheep fed alfalfa was recovered; however, when PEG was added to rumen contents from sheep fed either 40% or 100% CSH, recovery was decreased as CSH increased. There was also a trend for decreased recovery with time of incubation with CSH but not alfalfa. The in vitro studies indicated that CSH were adsorbing PEG at approximately 109 mg of PEG per 1.0 g of CSH.


FOOTNOTES

1 Contribution from the Missouri Agricultural Experiment Station. Journal Series 6229. Approved by the Director

2 Present address: Department of Animal Science, Louisiana State University, Baton Rouge.

3 Present address: Department of Animal Science, The Ohio State oniversity, Wooster, Ohio 44691.







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