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Na l-¹⁴C Stearate and Na 2-¹⁴C Acetate Metabolism and Morphological Analysis of Late Prepartum Bovine Mammary Tissue.

Department of Food Science, Cornell University, Ithaca, New York 14850
Department of Dairy Science, Virginia Polytechnic Institute and State University, Blacksburg 24061

ABSTRACT

Nonlactating mammary tissue from two cows, approximately 1 to 2 weeks (A) and 2 days (B) prepartum, actively utilized Na 1-¹⁴C stearate and Na 2-¹⁴C acetate for lipid synthesis. Tissue B incorporated significantly more of the radioactive substrates into triglycerides than Tissue A. Tissue B possessed stearyl desaturase which was not detected in Tissue A. Tissue B utilized about six times more acetate for fatty acid synthesis than did Tissue A, and this was preponderantly acylated into triglycerides. Fatty acid synthetase from Tissue B synthesized short chain fatty acids. Microscopic analyses revealed that the alveoli of tissue B were distended, had a low epithelium and abundant lumenal protein and lipid tion and a taller epithelium. Tissue B had 30% more lumenal area than A whereas the reverse was true for connective tissue. Epithelial, adipose, and vascular tissue areas were similar in both cows. Cellular lipid inclusions were more abundant and larger in. B than in A. In some instances secreted lipid had coalesced to form a single droplet filling an alveolar lumen. Protein secretion was evident in B but not as apparent in A.

From the biochemical data and fatty acid analyses it was apparent that Tissue B synthesized lipids typical of functional secretory tissue whereas A synthesized lipids similar to dedigerentiated or nonfunctional mammary tissue.

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