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Isoelectric Focusing of Bovine Colostrum Immunoglobulin¹

Department of Food Science and Nutrition, The Ohio Agricultural Research and Development Center and The Ohio State University, Columbus 43210

ABSTRACT

Isoelectric focusing in acrylarnide gels and sucrose gradient columns was used to study the heterogeneity of bovine colostrum immunoglobulins. The immunoglobulins were focused into a number of individual protein species with isoelectric points of from pH 4.6 to 6.7. Protein species exhibiting different isoelectric points also exhibited heterogeneity by immunoelectrophoresis. Re-focusing of the individual column fractions indicated that each major isoelectric protein was a homogeneous species and not an artifact. Individual classes and subclasses of the immunoglobulins were not identified.

¹ Approved as Journal Series Article 9–72 of the Ohio Agricultural Research and Development Center, Wooster. This investigation was supported in part by Public Health Services Grant EF-00161 from the Office of Research and Training Grants, Food and Drug Administration.