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Microbial Protein Biosynthesis in the Rumen

Department of Animal Science, University of Manitoba, Winnipeg, Canada

ABSTRACT

Semipurified and conventional diets with and without diethylstilbestrol (DES) were fed to four rumen fistulated dairy cows in a 4 x 4 Latin square design. Feed was limited to 10 kg daily and fed at intervals of 2 min. Amino acid composition of experimental diets, protozoa, bacteria, and rumen digesta were determined. Erperimental diets did not affect (P< .05) amino acid composition of the bacterial protein. Lysine, proline, phenylalanine, and methionine content of protozoa were affected (P<.05) by the experimental diets.

Diaminopimelic acid of bacteria and diaminoethylphosphonic acid of protozoa were used as markers to estimate microbial protein synthesis in the rumen. Rumen microorganisms contributed 54.4 to 91.8% of the total amino acids in rumen digesta with 24.3 to 30.9% in the form of bacterial amino acids. Protozoal amino acids as a percentage of the total rumen amino acids were increased more than twofold with both types of diet when DES was included in the diet.

Total amounts of amino acids passing to lower gut with lignin as a marker were 882 and 937 g daily when the semipurified diets without and with DES were fed and 1,108 and 1,271 g daily when the conventional diet without and with DES was fed.

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				J. Boguhn, H. Kluth, and M. Rodehutscord Effect of Total Mixed Ration Composition on Amino Acid Profiles of Different Fractions of Ruminal Microbes In Vitro J Dairy Sci, May 1, 2006; 89(5): 1592 - 1603. [Abstract] [Full Text] [PDF]