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Cerebral Ventricular Injections of Pentobarbital, Glucose, and Sodium Chloride into Sheep and Calves, and Feeding^1,2,

Animal Nutrition Laboratories, Department of Animal Science, Pennsylvania State University, University Park, Pennsylvania 16802
and Department of Nutrition, Harvard School of Public Health, Boston, Massachusetts 02115

ABSTRACT

Experiments determined if areas of ovine and bovine brain can be inactivated, thereby releasing the inhibition on eating in a satiated animal. Pentobarbital sodium, a neural depressant, was injected into a lateral cerebral ventricle to test this possibility. Glucose and NaCl solutions of osmolality (2,650 milliosmoles/kg H₂0) similar to the pentobarbital were also tested. Eating began within 7 min after injection of 29.4 mg of pentobarbital and continued intermittently for averages of 27 and 32 min in sheep and calves. Average food intake of sheep (18 observations) for the half-hour following pentobarbital injection versus the same time on non-injection days was 344.5 ± SE. 44.8 g versus 42.8 ± 6.6 g (P<.001). Growing calves also showed an increase (P<.001) in food intake for 30 min following pentobarbital injection (9.37 ± .73 g/kg versus 1.23 ± .18 g/kg). A 32% glucose solution (.45 ml) injected into the lateral ventricle of sheep resulted in variable responses but generally did not increase food intake. The same amount of glucose injected into a lateral ventricle of calves, the injection made over 5.5 min, increased food intake above noninjection periods (P<.05). In sheep, ventricular injections of sodium chloride solution induced drinking within 7 min but did not induce eating. We propose that the eating response after injection of pentobarbital into a lateral ventricle of sheep and calves is evidence for inhibitory action of areas in the brain, probably in the medial hypothalamus.

¹ Authorized for publication 4-28-71 as paper 3967 in the journal series of the Pennsylvania Agricultural Experiment Station.

² This work was supported in part by a TJ. S. Public Health Service Grant AM 12023 from the National Institute of Arthritis and Metabolic Diseases and the Fund for Eesearch and Teaching, Department of Nutrition, Harvard School of Public Health.

³ Current address: Smith, Kline and French Laboratories, 1600 Paoli Pike, West Chester, Pennsylvania 19380.