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Methionine Stimulation of Milk Protein Synthesis in Bovine Mammary Cell Cultures¹

Department of Dairy Science, University of Illinois, Urbana, Illinois 61801

ABSTRACT

Dispersed cell cultures of lactating bovine mammary tissue with a complete culture medium were tested for their ability to synthesize milk proteins ß-lactoglobulin and ß-casein at elevated levels of methionine. An effect showed up to a L-methionine concentration of 50 to 100 µg/ml which increased synthesis of the proteins to two-fold over that with a L-methionine of about 15 µg/ml in bovine blood serum and in the usual cell culture medium. Further increases resulted in an apparent inhibitory effect. The effect suggests that L-methionine normally in blood serum and used in cell culture medium may be deficient for maximum milk protein synthesis.

¹ This work was supported in part by grants from the National Science Foundation and by the Illinois Agricultural Experiment Station.