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Lactational Trends in Vitamin B₁₂ Status on Conventional and Restricted-Roughage Rations

Department of Animal Science, Cornell University Ithaca, New York 14850

ABSTRACT

Sixteen Holstein cows were assigned to one of two dietary treatments, control or restricted-roughage, for the first 34 weeks of lactation. Vitamin B₁₂ in rumen fluid, blood serum, liver, milk and urine was monitored at intervals to determine general trends and to test the hypothesis that roughage restriction would alter the B₁₂ status of animals on this treatment. The per cent of total vitamin B₁₂ activity in rumen fluid represented by the vitamin itself was reduced by roughage restriction. This treatment resulted in higher serum, lower liver and milk and higher urine vitamin B₁₂ activity. Lactational trends are described. Interrelationships among variables suggested the possibility that higher serum B₁₂ of cows on restricted roughage might be due in part to vitamin B₁₂ analogues.

¹ Present address: Department of Animal and Veterinary Sciences, University of Maine, Orono 04473;

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