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Effect of Some Zwitter Ion Buffers on the Freezing and Storage of Spermatozoa I. Bull¹

Department of Animal Science, University of Minnesota, St. Paul 55101

ABSTRACT

Seven zwitter ion buffers titrated with bases, Tris (hydroxymethyl) amino methane (Tris), NaOH, and combinations of NaOH and KOH to pH 7.0 along with Tris titrated with HCl, sodium citrate, and phosphate were prepared. The effect of these buffers on motility and glutamic oxalacetic transaminase (GOT) of fresh bull spermatozoa, of spermatozoa stored at 37 C for 6 hr and at 5 C for 48 hr and after freezing was studied.

Spermatozoa diluted in zwitter ionic buffer were more motile but released more GOT than sperm diluted in phosphate or citrate.

N-Tris (hydroxymethyl) methyl-2-amino ethane sulfonic acid (TES) with Tris as the titration base was the most satisfactory buffering system for diluting bull spermatozoa for freezing.

¹ Scientific Journal Series Paper 7446, Minnesota Agricultural Experiment Station.

² Present address: Department of Poultry-Science, Ohio Agricultural Research and Development Center, Wooster 44691.