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Stability of Methionine Hydroxy Analog in Rumen Fluid and Its Conversion in Vitro to Methionine by Calf Liver and Kidney

Research Division, Biochemicals Department, E. I. du Pont de Nemours & Co., Inc., Wilmington, Delaware 19898

ABSTRACT

In vitro rumen studies with methionine hydroxy analog-1-¹⁴C (M-analog) and L-methionine-1-¹⁴C indicate that the analog is much more resistant to rumen microbial degradation. The disappearance of ¹⁴C-methionine was associated with the formation of large amounts of ¹⁴CO₂ and increase in ¹⁴C-activity of the rumen solids, the latter being indicative of incorporation of methionine or its metabolites or both into bacterial cellular components. The disappearance of ¹⁴C-M-analog, in contrast, was associated with ¹⁴CO₂ formation but not with bacterial cell incorporation.

Incubation of ¹⁴C-M-analog with calf liver and kidney microsomal fractions revealed that these enzyme systems were equally capable of converting the analog to methionine. Glutamine, leucine, and asparagine were comparable as amino group donors.

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