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Journal of Dairy Science Vol. 55 No. 2 272-274
© 1972 by American Dairy Science Association ®
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Gas Chromatographic Determination of Methionine Hydroxy Analog in Rumen Fluid

Nicholas J. Alicino and Stanley E. Katz

Diamond Shamrock Chemical Company, Harrison, New Jersey 07029
, and Department of Biochemistry and Microbiology, Rutgers The State University of New Jersey, New Brunswick 08903

ABSTRACT

Methionine hydroxy analog was determined in rumen contents after extraction of acidified contents with ethylacetate and reaction with a silylating reagent to form a trimethylsilane ester which was measured gas chromatographically. Recoveries averaged 76.9% in rumen fluid, 76.5% for centrifuged rumen fluid, and 75.7% for screened rumen fluid in the range of 10 to 480 µg/g.






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Copyright © 1972 by the American Dairy Science Association ®.