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Journal of Dairy Science Vol. 55 No. 2 194-199
© 1972 by American Dairy Science Association ®
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Streptococcus uberis of Bovine Mastitis Origin: Isolation, Characterization, and Serology of Two Mucoid Strains

B. Misra1 and R. T. Marshall

Department of Food Science and Nutrition, University of Missouri, Columbia 65201

ABSTRACT

Thirty-three per cent of the quarters in a private herd of 30 Holstein cows that had severe clinical mastitis produced visibly abnormal milk at one milking. Milk samples from this herd taken 14 months apart yielded two mucoid strains, U3M and U5M, biochemically typical of Streptococcus uberis. Of 8 samples taken from glands infected solely with Streptococcus uberis, seven had standard plate counts of at least 390,000 per milliliter. Significant differences were noticed between the 2 mucoid strains. U3M did not ferment melezitose, was CAMP positive in TKT medium, grew in 4% NaCl, and grew at temperatures of 21 and 45 C, but U5M possessed opposite characteristics. Two nonmucoid strains were selected from the mucoid strains by passage in laboratory media. There were no differences in biochemical and physiological characteristics between the mucoid strains and their own nonmucoid variants. Cross-agglutination tests with hyperimmune sera indicated a definite relationship among the two mucoid strains and their nonmucoid variants. However, a high titer was noted only for the homologous antiserum of the U5M strain. Autoagglutination was an important problem. Presence of group specific precipitating antibody could not be demonstrated by the capillary tube precipitin or gel-diffusion tests. The three strains tested failed to react with any of the Lancefield group sera. Three strains of S. uberis in pathogenicity trials were avirulent for laboratory mice.

FOOTNOTES

1 Present address: Orissa Agriculture University, Bhubaneswar 3, Orissa, India.






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Copyright © 1972 by the American Dairy Science Association ®.