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Determination of Rennin and Bovine Pepsins in Commercial Rennets and Abomasal Juices

Centre National de Recherches Zootechniques, Laboratoire de Recherches sur les Proteines INRA, 78, Jouy en Josas, France

ABSTRACT

A DEAE cellulose column chromatographic method is described to separate and to titrate rennin and bovine pepsin II from a mixture. Microgranular DEAE cellulose was equilibrated in .02 M piper-azine buffer pH 5.3 and was placed on a 1 x 8 cm column. The enzyme solution was poured on the column, as such, or, if necessary, after dialysis against the same buffer. Elution of enzymes was by a linear gradient of NaCl (100 ml of .15 M NaCl in the first chamber and 100 ml of .5 M NaCl in the second chamber). The flow was about 80 ml per hour. Rennin was eluted in the first fraction and pepsin II in the second fraction. This method was used to determine the enzymatic composition of several commercial rennet preparations and abomasal juices collected from fistulated calves.

¹ In partial fulfillment of the requirements for the degree of Doctour 3é cycle, Faculté des Sciences, Orsay, France, 1972.

² Present address: Laboratoire de Recherches de la Chaire de Zootechnie, INRA, 65 rue de St Brieue, 35, Rennes, France.

³ Present address: Laboratoire de Recherches de Technologie Laitière, INRA, 65 rue de St Brieue. 35, Rennes, France.

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