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Journal of Dairy Science Vol. 55 No. 11 1577-1585
© 1972 by American Dairy Science Association ®
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Membranes of Mammary Gland. V. Isolation of Golgi Apparatus and Rough Endoplasmic Reticulum from Bovine Mammary Gland

T. W. Keenan, C. M. Huang and D. James Morre

Departments of Animal Sciences, Botany and Plant Pathology and Biological Sciences, Purdue University, Lafayette, Indiana 47907

ABSTRACT

Methods are presented for isolation of Golgi apparatus and ribosome-studded (rough) endoplasmic reticulum fractions from bovine mammary gland in quantities and purity sufficient for biochemical analyses. These fractions were characterized morphologically and enzymologically. Galactosyl transferase and NADPH1-cytochrome c reductase were suitable marker enzymes for Golgi apparatus and endoplasmic reticulum, respectively, from bovine mammary tissue.






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Copyright © 1972 by the American Dairy Science Association ®.