JDS
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Journal of Dairy Science Vol. 55 No. 10 1508-1510
© 1972 by American Dairy Science Association ®
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Josephson, R. V.
Right arrow Articles by Sinha, D. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Josephson, R. V.
Right arrow Articles by Sinha, D. P.

Gel Isoelectric Focusing of Selected Bovine Immunoglobulins1

R. V. Josephson, E. M. Mikolajick and D. P. Sinha

Department of Food Science and Nutrition, The Ohio State University, Columbus 43210

ABSTRACT

Commercial IgG1, IgG2, and IgM bovine immunoglobulin fractions were relatively homogeneous by acid polyacrylamide gel-electrophoresis and immunoelectrophoresis. However, isoelectric focusing in polyacrylamide gels with ampholytes covering wide and narrow pH gradients revealed heterogeneity among IgGl and IgG2 species with estimated isoelectric points in the pH ranges of 5.5 to 6.8 and 7.5 to 8.3, respectively. IgM was not resolved by gel isoelectric focusing. IgGl and IgM were tentatively identified as the major and minor immunoglobulin components, respectively, of bovine colostrum whey.

FOOTNOTES

1 Article 3:72. This investigation was supported by Public Health Services Grant FD-00161 from the Office of Research and Training Grants, Food and Drug Administration.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1972 by the American Dairy Science Association ®.