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Journal of Dairy Science Vol. 55 No. 10 1506-1507
© 1972 by American Dairy Science Association ®
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Discontinuous Polyacrylamide Electrophoresis for ß-Lactoglobulin Typing of Cow's Milk

C. A. Kiddy, R. E. Rollins and J. P. Zikakis

Animal Science Research Division, Agricultural Research Service, USDA, Beltsville, Maryland 20705
, and Department of Animal Science and Agricultural Biochemistry, University of Delaware, Newark, Delaware, 19711

ABSTRACT

The use of a spacer gel, in vertical polyacrylamide gel electrophoresis for ß-lactoglobulin typing, gives better results than previous methods where only one gel concentration was used. The method is a modification of that in Technical Bulletin 140 of the E-C Appartus Corporation. The advantages of the method are speed, improved resolution, economy (cost of chemicals reduced by 50%), and dependability.






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