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Elemental Sulfur: Degradation Product of Thiamine in Model Systems¹

The nature of heat-induced thiamine degradation in an aqueous medium was investigated. Thiamine hydrochloride solutions were buffered to slightly acid and to slightly basic pH and were heated at 121 C for 40 min. Degradation products were extracted with benzene and separated by thin-layer chromatography. A characteristic degradation pattern was observed, with a major degradation product being identified as elemental sulfur.

¹ Journal Paper 4476 of the Purdue University Agricultural Experiment Station.