HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Richter, R. L. Articles by Randolf, H. E. Search for Related Content PubMed PubMed Citation Articles by Richter, R. L. Articles by Randolf, H. E.

Purification and Properties of a Bovine Milk Lipase

Department of Animal Science, Texas A&M University, College Station 77843

ABSTRACT

A low molecular weight lipase was isolated from clarifier slime. It was precipitated from a water extract of an aceton powder prepared from clarifier slime at 20 to 45% of saturation with (NH₄)₂SO₄ and was purified by ultrafiltration. Final isolation was with Sephadex G-75. Estimated molecular weight was 8,500 for the enzyme which contained 7.6% carbohydrate. The enzyme exhibited maximum activity at pH 9.2 and 37 C. It hydrolyzed a simple short-chain fatty acid triglyceride faster than long-chain fatty acid triglycerides, but had little specificity for natural oil emulsions. Enzyme activity rapidly decreased at 37 C and only 16% of the original activity existed after 5 hr. Storage for 48 hours at 23 and at 4 C decreased lipase activity 75 and 23% with complete inactivation after 1 hour at 45 C.

¹ Present address: Department of Food Science and Industries, University of Minnesota, St. Paul 55101.