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Metabolism of Uniformly C ¹⁴ -Labeled Fructose, Sorbitol, and Glycerol in Lactating Goats¹

Department of Physiological Sciences, School of Veterinary Medicine, University of California, Davis 95616

ABSTRACT

This investigation compared utilization of C¹⁴ -fructose with C¹⁴-sorbitol, and C¹⁴-glycerol after intravenous injection (pulse-label) of each substance into lactating goats.

Autoradiograms of plasma samples showed rapid removal of fructose and early appearance of C¹⁴ in glucose. The initial half-life for disappearance of fructose glycerol from plasma was less than three minutes. Plasma glucose reached maximum specific activity in approximately 10 min following injection of each compound. Calculations showed that, 54% of the glycerol, 60% of the fructose, and 64% of the sorbitol had entered the plasma glucose pool during three hours after pulse labeling.

A smaller amount of each compound, about 30% of that injected, was oxidized to CO₂ but nearly four-fifths of this appeared in glucose prior to oxidation. The respired CO₂ reached maximum specific activity between 45 and 55 minutes after injection of fructose or sorbitol and 22 minutes after injection of glycerol. The magnitude of the peak specific activity after glycerol injection was nearly twice that reached with either fructose or sorbitol.

More of the glycerol was oxidized and less was converted to glucose than for either fructose or sorbitol.

¹ Supported by U.S. Public Health Service Research Grant HD-02375. Taken from a thesis submitted by the senior author in partial fulfillment of the requirements for the Ph.D. Degree University of California.

² Recipient of International Atomic Energy Agency Fellowship. Present address: Department of Physiology and Pharmacology, School of Veterinary Medicine, Bogor Agricultural University, Bogor, Indonesia.