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Fatty Acid Esterification by Homogenates of Bovine Liver and Adipose Tissue¹

Dairy Department, Michigan State University, East Lansing 48823

ABSTRACT

Glycerol-3-phosphate, adenosine triphosphate, coenzyme A, MgCl₂, and NaF were necessary cofactors for maximal esterification of palmitate-1-¹⁴ by homogenates of bovine liver and adipose tissue. Dithiothreitol enhanced esterification in liver, but not in adipose tissue homogenates, whereas esterification was stimulated by bovine serum albumin in adipose but not hepatic tissue. Both systems incorporated palmitate, stearate, oleate, and linolenate into glycerides. Linoleate incorporation was scant by either system. Liver homogenates esterified a greater percentage of the fatty acids into phospholipids and monoglycerides than did adipose tissue. Triglycerides accounted for 55% of the product in adipose tissue and 18 to 44% in liver.

¹ This work was included in a thesis submitted by the first author to the Dairy Department and Institute of Nutrition, Michigan State University, in partial fulfillment of the requirements for the degree of Doctor of Philosophy. Partially supported by NIH Training Grant GMO-1818, Research Grant AM 13177 and published as Michigan Agricultural Experiment Station Journal Article 5309.

² Present address: Letterman Army Institute of Research, San Francisco, California 94129.