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Journal of Dairy Science Vol. 54 No. 6 835-841
© 1971 by American Dairy Science Association ®
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Purification of the Protein of Human Milk Lactose Synthetase and Identification as a Glycoprotein

T. Nagasawa, I. Kiyosawa and N. Tanahashi

Central Research Laboratory, Morinaga Milk Industry Company, Ltd., Tokyo, Japan

ABSTRACT

The A protein of human milk lactose synthetase (UDP-d-galactose : d-glucose 1-galaotosyltransferase EC 2.4.1.22) was purified and investigated. The A protein contained hexose (galactose, mannose fucose), hexosamiie (galactosamine and glucosamine) and sialic acid. Thus the A protein was identified as a glycoprotein enzyme. Amino acid composition determined that proline and glutamic acid were very high. Molecular weight of the A protein was estimated at 72,000 to 75,000 by gel filtration and electrophoretic mobility.




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Copyright © 1971 by the American Dairy Science Association ®.