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Reclamation of Whey Protein with Carboxymethylcellulose

Department of Dairy Technology, The Ohio State University, Columbus 43210

ABSTRACT

Molecular complex formation between anionic hydrocolloids and proteins was used to recover proteins from whey with carboxymethylcellulose. A predetermined amount of the hydrocolloid was mixed with acidifled whey and the resulting complex removed by centrifugation. More than 90% of the protein was recovered in an acidic fraction (Fraction I ) at pH 3.2. Following removal of this complex, additional protein was precipitated in an alkaline fraction (Fraction II) at pH 7.5 to 8.0. After dehydration, Fraction I contained about 60% protein and 30% carboxymethylcellulose. Fraction II was composed predominantly of calcium phosphate, containing only about 15% protein and 4% of the cellulose. Electrophoresis showed that most of the whey proteins were in Fraction I whereas Fraction II contained proteose peptones. Fraction I was soluble in water by adjusting the pH to 7 to 7.5 whereas Fraction II was soluble at pH 5.5 and below.