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Orange G Dye Binding for Determination of Protein of Sow's Milk¹

Department of Animal Sciences, Purdue University, Lafayette, Indiana 47907

ABSTRACT

Equations for protein in sow's milk by Orange G dye binding procedures were: per cent protein = 9.184-4.526 (absorbance) at Sensitivity 1 for samples above 6.5% protein, and per cent protein = 7.317-6.061 (absorbance) at Sensitivity 2 for samples containing 3.0 to 6.5% protein. Forty-two samples of sow's milk were analyzed with a Beckman Model B spectrophotometer. Sow's milk had more protein than cow's milk, requiring 1 ml milk per 25 ml Orange G dye. Protein analyses by Orange G dye binding were 0.95 correlated with Kjeldahl estimates of protein. These equations differed from those developed for estimating protein of cow's milk by the Orange G procedure.

¹ Journal Paper 4112 of the Purdue University Agricultural Experiment Station, Lafayette, Indiana 47907.