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Fertility of Bull Semen with Added Beta-Glucuronidase¹

Dairy Department, Michigan State University, East Lansing 48823

ABSTRACT

Motility and fertility of bull sperm were determined following storage in semen extender containing ß-glucuronidase. ß-glucuronidase retarded loss of sperm motility during 7 days storage at 5 C. A preliminary trial revealed no harmful influence of ß-glucuronidase on fertility of 279 cows inseminated with frozen semen.

Consequently, 120 ejaculates from 14 bulls were used to determine fertility of frozen semen extended in yolk-citrate-glycerol with 0, 10, 30, or 150 units of added ß-glucuronidase, or with 10 or 20 µg of added -amylase per milliliter. Bach enzyme treatment was replicated three times. The 60- to 90-day nonreturn percentages and numbers of first services (in parentheses) for 0, 10, 30 and 150 units of ß-glucuronidase were 72.0 (3,401), 72.3 (1,961), 73.2 (1,912) and 77.9 (1,734). Fertility for 150 units was greater than that for 10 and 30 units of ß-glucuronidase (P 0.02). Comparable values for 10 and 20 µg of -amylase per milliliter of semen extender were 74.3 (1,504) and 74.2 (2,057).

¹ Michigan Agricultural Experiment Station Journal Article 5264. Supported by XJSPHS Research Grant HD 03039.

² Select Sires, Inc., East Lansing, Michigan 48823.

³ Department Animal Science, University of Minnesota, St. Paul 55101.