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Department of Animal Products Technology, Faculty of Agriculture, Okayama University, Tsushima, Okayama, Japan
ABSTRACT
A temperature-gradient incubator consisting of an aluminum block with pairs of 30 holes was used for investigating temperature-related anomalies in the growth of Streptococcus lactis, Lactobacillus casei, Lactobacillus bulgaricus, Bacillus sp. and Pseudomonas sp. Multiple temperature minima for the bacteria grown for 18 to 20 hr in yeast-glucose-peptone broth or in skimmilk occurred at 16 to 17, 29 to 32, and 43 to 44 C. The optima occurred at 14 t o 16, 28 to 30, 32 to 42, and 44 to 45 C. The results are discussed for specific temperatures at which anomalous changes occur in the structure of water and aqueous solutions.
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