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Journal of Dairy Science Vol. 54 No. 11 1699-1701
© 1971 by American Dairy Science Association ®
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Separation of interfering Compounds in the Determination of Tocopherol in Milk

E. Low and W. L. Dunkley

Department of Food Science & Technology, University of California, Davis 95616

ABSTRACT

The method includes isolation of milk lipids by extracting a milk-ethanol-HCl mixture with hexane, chromatographing the extract on alumina to remove interfering compounds, and determining the tocopherol by a modified Emmerie-Engel reaction. Results illustrate the influence of selected experimental variables on the separation of interfering compounds from tocopherol and the recovery of tocopherol, and compare analyses by the new method and the two described previously.






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Copyright © 1971 by the American Dairy Science Association ®.