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Journal of Dairy Science Vol. 54 No. 10 1536-1540
© 1971 by American Dairy Science Association ®
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Aging Phenomena in Spermatozoa. V. Thermal Denaturation Characteristics of Deoxyribonucleic Acid Isolated from Fresh and Aged Bovine Spermatozoa1

M. Berchtold2, G. W. Salisbury and C. N. Graves

Department of Dairy Science, University of Illinois, Urbana 61801

ABSTRACT

The mean denaturation temperature or melting point of DNA isolated from freshly ejaculated bovine spermatozoa was 86.43 ± .25 C with an average increase in optical density upon heat denaturation of 37.8 ± 1.3%. The average atomic extinction coefficient for phosphorus was 6,350. Neither storage of spermatozoa for up to 15 days at 4 C or 100 days at –79 C nor X-irradiation of the spermatozoa followed by storage for up to 8 days prior to extraction of the DNA significantly altered either the denaturation temperature or the increase in optical density from heat denaturation. The melting point of heat denatured spermatozoan DNA was 44.4 C. The ionic strength of the medium used to suspend the DNA preparation also influenced the melting profile with a reduction in ionic strength of the medium, shifting the melting point toward lower temperatures.

FOOTNOTES

1 Supported in part by NTH Grant RG-8753 TJSPHS and by Hatch Project 30-317 from the Illinois Agricultural Experiment Station.

2 Present address: Gynaekologische Tierklinik der TJniversitaet Munehen, Koeniginstrasse 12. Germany.






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Copyright © 1971 by the American Dairy Science Association ®.