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Section of Biochemistry, Department of Life Sciences, Bar-llan University, Ramat-Gan, Israel
ABSTRACT
The milk coagulating enzyme of Mucor pusillus compared to crude calf rennet was stimulated similarly by aluminium, ferric, calcium, and magnesium ions. The tri-valent ions were more effective than the divalents. At low ion concentrations, the addition of Mucor pusillus enzyme to calf enzyme greatly increased activity as compared to the activity in separate preparations. Therefore, one enzyme system appears to be a synergist to the other, suggesting two separate coagulation mechanisms.
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