HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Shultz, T. A. Articles by Shultz, E. Search for Related Content PubMed PubMed Citation Articles by Shultz, T. A. Articles by Shultz, E.

Estimation of Rumen Microbial Nitrogen by Three Analytical Methods

Facultad de Ciencias Veterinarias, Universidad Central de Venezuela
and Centro de Investigaciones Veterinarias, Ministerio de Agricultura y Cria, Maracay, Venezuela

ABSTRACT

Perchloric acid-soluble residue, tungstic acid precipitate, and separately funnel techniques were evaluated by comparing results of microbial cell dry matter, per cent nitrogen, and total nitrogen in rumen samples before and after feeding. Significantly less (P 0.01) cellular dry matter remained in perchloric acid samples than with other methods due to procedural complications in washing and decanting specimens. As a result of incomplete separation by the separatory funnel techniques, per cent nitrogen in the dried microorganisms was significantly reduced (P 0.01); however, microbial preparations by the separatory funnel method contained less (P 0.10) feed contamination than nonseparatory funnel procedures. The tungstic acid method consistently yielded significantly greater (P 0.01) amounts of total nitrogen from the rumen microbial samples, suggesting that limitations in the other methods were of importance in quantitative analyses.