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Journal of Dairy Science Vol. 53 No. 5 599-601
© 1970 by American Dairy Science Association ®
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Simplified Pouch Method for Enumeration of "Stinker" Swiss Cheese Organisms1

G. H. Richardson and G. A. Fowler

Department of Food Science and Industries, Utah State University, Logan 84321
Cache Valley Dairy Association, Smithfield, Utah 84335

ABSTRACT

Clostridium species have been successfully enumerated in a rectangular, commercially available boil-in-bag pouch. One milliliter of raw milk and 45 ml of Te++-containing dextrose-free thioglycollate medium are sealed in the pouch, heated for 10 minutes at 80 C, and allowed to solidify. Hydrogen sulfide—producing colonies are enumerated after 48 hours at 37 C.

FOOTNOTES

1 Contribution from the Utah Agricultural Experiment Station, no. 953. Approved by the director.






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