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Lipid Synthesis by Rumen Microorgnisms. II. Further Characterization of the Effects of Methionine^1,2,

Lipids Laboratory, Department of Dairy Science, Pennsylvania Agricultural Experiment Station, University Park 16802

ABSTRACT

Characteristics of the lipids formed by rumen microbes as a result of methionine stimulation were investigated. Tracer studies with acetate, glucose, and long-chain fatty acids revealed substantial transfer of carbon from these sources to complex microbial lipids. The extensive labeling of a variety of lipid compounds, and especially the stimulation of polar lipid formation, suggests that the lipids formed were serving as structural components related to microbial growth. It is believed that most of this new lipid was associated with rumen protozoa.

¹ Authorized for publication as paper no. 3701 in the Journal Series of the Pennsylvania Agricultural Experiment Station on November 24, 1969.

² Supported in part by The Pennsylvania Department of Agriculture and U.S. Public Health Service Grant HE 03632.

³ Supported by Ralston Purina fellowship.