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Kinetics of N-Ethylmaleimide Inhibition of L-Alanine-Induced Germination of Bacillus cereus Spores¹

Department of Dairy Technology, The Ohio State University, Columbus 43210

ABSTRACT

The Kinetics of inhibition of l-alanine-induced germination of Bacillus cereus 7 spores by the sulfhydryl blocking agent, n-ethylmaleimide, were investigated. The germination response of heat-activated spores in 0.1, 0.01, 0.003, 0.002, and 0.001 m concentrations of l-alanine (substrate) and 0.001 and 0.003 m concentrations of the inhibitor was determined at a constant temperature of 35 C. The velocity, fraction of the germination reaction completed, and per cent inhibition of the spore germination were calculated. The velocity and fraction of the germination reaction completed were found to have a direct relationship to the substrate concentration at all inhibitor levels. The inhibition of l-alanine-induced spore germination varied directly with n-ethylmaleimide concentration. The Lineweaver-Burk plot indicated that the inhibition was competitive. The l-alanine concentrations required for B. cereus 7 spores in 0, 0.001, and 0.003 m of n-ethylmaleimide were calculated to be 0.303, 0.476, and 2.5 m, respectively.

¹ Article no. 1-69, Department of Dairy Technology. This research was supported by Public Health Services Research Grant FD-00072 from the Office of Reseach and Training Grants, Food and Drug Administration.

² Present address: Argonne Cancer Research Hospital, Illinois 60637.