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Characterization of Diacetyl Negative Mutants of Streptococcus diacetilactis¹

Department of Microbiology, Oregon State University, Corvalli 97331

ABSTRACT

A procedure for the mutation of lactic streptococci with n-methyl-n'-nitro-n-nitro-soguanidine was used to isolate nondiacetyl producing mutants of Streptococcus diacetilactis. The mutants produced an average of 40% more lactic acid than parent strains; acetaldehyde production varied from quantities equal to the wild type to less than one-third the amount produced by the parents. The mutants possessed a citrate transport system catalyzing the accumulation of citrate at a rate which diminished with time. The diacetyl negative mutants retained inhibitory powers against food spoilage bacteria similar to their respective parents.

¹ Technical Paper no. 2775, Oregon Agricultural Experimen Station, Corvallis.