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Growth of Selected Bacteria in Liquid Media Containing DDT, Dieldrin and Heptachlor¹

Department of Animal Sciences, Food Science Section, University of Kentucky, Lexington 40506

ABSTRACT

Growth of Pseudomonas fluorescens and Staphylococcus aureus in trypticase soy broth was affected by DDT exceeding 50 µg per milliliter. This effect was more pronounced after the first day of incubation. No effect on growth was observed in skimmilk containing up to 100 µg per milliliter of DDT.

Concentration of 3 µg per milliliter of heptachlor in trypticase soy broth inhibited the growth of S. aureus up to 18 hours, whereas 15 to 20 µg per milliliter prevented growth up to 7 days. Inhibition of S. aureus was not observed in skimmilk or in trypticase soy broth plus 3% whole casein or mixture of - and ß-casein fraction containing up to 100 µg per milliliter of heptachlor. Heptachlor epoxide had no inhibitory effect, whereas 72% heptachlor was more inhibitory than 99% heptachlor.

¹ Published with the approval of the Director of the Kentucky Agricultural Experiment Station as journal article no. 70-5-81.

² Present address, Department of Animal Science, University of Arkansas, Fayetteville 72071.