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Determination of Contained-Milk Volume of the Mammary Gland of the Goat by ¹⁴¹Cerium and ¹⁴C-Peg-4000¹

Department of Physical Biology, New York State Veterinary College, Cornell University, Ithaca, New York 14850

ABSTRACT

Cerium-141 and ¹⁴C-polyethylene glycol, molecular weight 4,000, were used in an isotope dilution technique to determine the volume of milk in the mammary gland of the goat. Procedures included introduction of label into the mammary gland by way of the teat meatus and an intravenous injection of 2 IU of oxytocin followed by hand manipulation of the udder to facilitate uniform mixing of cistern, duct, and that portion of the alveolar milk let down in response to the oxytocin. Assay of any size milk sample taken after this procedure allows approximation of the mammary gland—contained milk volume at the time of the oxytocin dose.

Results were consistently the same for both ¹⁴¹Ce and ¹⁴C-polyethylene glycol, allowing the option of using either label in the technique. Recover}' of both labels was essentially 100% 3 days after dosing the udder, and no ¹⁴¹Ce was detected in blood or urine within 24 hours. Label was distributed uniformly among serial samples of the milk readily collectable after the mixing procedure, representing 87.9% of the computed total milk volume. A portion of the residual milk recovered with a second dose of oxytocin showed ¹⁴¹Ce and ¹⁴C-polyethylene glycol amounts similar to those of the milk already collected.

¹ Supported in part by U.S. Atomic Energy Commission under Contract AT(30-1)-4039.