HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Askew, E. W. Articles by Thomas, J. W. Search for Related Content PubMed PubMed Citation Articles by Askew, E. W. Articles by Thomas, J. W.

Lipoprotein Lipase of the Bovine Mammary Gland^1,2,

Dairy Department, Michigan State University, East Lansing 48823

ABSTRACT

Homogenates of lactating and nonlactating bovine mammary tissue were examined for lipoprotein lipase (EC 3.1.1.3) activity to provide information on the mode of uptake of serum triglyceride fatty acid by the bovine mammary gland. The activity of lipoprotein lipase in lactating bovine mammary gland homogenates was dependent upon the concentrations of bovine serum albumin, serum, and pH of the incubation mixture. The cation Ca⁺⁺ did not stimulate bovine mammary tissue lipolytic activity. Heparin caused minor but variable degrees of stimulation (3 to 25%) of lipolytic activity. The majority (80%) of cellular lipolytic activity was associated with the particulate fraction. Lipoprotein lipase activity was similar in all quarters of the udder, unaffected by freezing, and greatly reduced in nonlactating tissue. Milk contained a lipase with properties similar to tissue lipoprotein lipase.

¹ Published with the approval of the Director of the Agricultural Experiment Station as Journal Article no. 5022.

² Partially supported by NIH Training Grant GMO-1818 and Research Grant AM13177.

³ Present address: United States Army Medical Research and Nutrition Laboratory, Fitzsimons General Hospital, Denver, Colorado 80240.